The match between the two central unions, which is purely for bragging rights, will kick off at Tafel Lager Park at 16h30, and will be broadcast live on SuperSport.
Mathie tweaked the squad that came up short in Durban last weekend as they hunt their first and only win in the competition.
Daniel Kasende will start on the right wing, with Harlon Klaasen on his inside, replacing Enver Brandt and Berton Klaassen respectively.
In the forward pack, Mathie recalled Adre Smith (lock) in place of Ewan Coetzee, who in turn starts among the replacements.
In the other change, prop Mox Mxoli, has been promoted to the starting team, switching with Andrew Beerwinkel, who is one of the impact players.
Mathie named an extended bench, featuring experienced flyhalf and former captain, George Whitehead, who missed a big chunk of the season due to injury, while Ederies Arendse (wing) comes in for Johnathan Francke.
Masixole Banda (utility back) could also get a run if he features in the finalised match day squad.
The Tafel Lager Griquas - 15 James Verity-Amm, 14 Daniel Kasende, 13 Harlon Klaasen, 12 Andre Swarts, 11 Eduan Keyter, 10 Tinus de Beer, 9 Zak Burger (Captain), 8 Johan Momsen, 7 Stefan Willemse, 6 Gideon van der Merwe, 5 Cameron Lindsay, 4 Adre Smith, 3 John-Roy Jenkinson, 2 Alandre van Rooyen, 1 Mox Mxoli. Replacements (from): 16 CJ Conradie, 17 Bandisa Ndlovu, 18 Tijde Visser, 19 Ewan Coetzee, 20 Siba Qoma, 21 Ashlon Davids, 22 George Whitehead, 23 Ederies Arendse, 24 Andrew Beerwinkel, 25 Raegan Oranje, 26 Masixole Banda.
Match information – Tafel Lager Griquas v Toyota Cheetahs
Date: Saturday, 9 January 2021
Venue: Tafel Lager Park, Kimberley
Referee: Aimee Barrett-Theron
Assistant Referees: Griffin Colby and Paul Mente
TMO: Cwengile Jadezweni
TV: SuperSport Grandstand and Rugby